Antigenic analysis of Barber's protein antigen prepared from Salmonella typhi and demonstration of protein-specific antibodies in sera of typhoid patients.


A crude protein antigen (Barber's antigen) was prepared from Salmonella typhi by mild extraction with veronal buffer followed by repeated precipitation with trichloracetic acid according to the method of Barber. From serological studies and polyacrylamide gel electrophoresis, Barber's antigen was found to be highly heterogeneous and contain both the… (More)