Antigen specificity and tumour targeting efficiency of a human carcinoembryonic antigen-specific scFv and affinity-matured derivatives.

@article{Jackson1998AntigenSA,
  title={Antigen specificity and tumour targeting efficiency of a human carcinoembryonic antigen-specific scFv and affinity-matured derivatives.},
  author={H. Jackson and L. Bacon and R. Pedley and E. Derbyshire and A. Field and J. Osbourn and D. Allen},
  journal={British Journal of Cancer},
  year={1998},
  volume={78},
  pages={181 - 188}
}
We have examined the biological properties of CEA6, a human carcinoembryonic antigen (CEA)-specific single-chain Fv (scFv) isolated by phage display, and five related clones derived by affinity maturation and selected for improved off-rate (Koff). All clones bind strongly and specifically to CEA-positive human tumours by immunocytochemistry and show negligible cross-reactivity with normal colon. Flow cytometry of scFv on human liver cells indicates a shift in fine epitope specificity resulting… Expand
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