Antigen specificity and activity of ovine antibodies induced by immunization with Corynebacterium pseudotuberculosis culture filtrate.

@article{Ellis1991AntigenSA,
  title={Antigen specificity and activity of ovine antibodies induced by immunization with Corynebacterium pseudotuberculosis culture filtrate.},
  author={John A. Ellis and D A Hawk and Kenneth W. Mills and Diana L. Pratt},
  journal={Veterinary immunology and immunopathology},
  year={1991},
  volume={28 3-4},
  pages={
          303-16
        }
}
Sheep were immunized three times with a vaccine composed of filtrate from a 36 h culture of Corynebacterium pseudotuberculosis and a block polymer adjuvant. Immunization resulted in the development of exotoxin-neutralizing antibody. This corresponded to the recognition of a 31.6 kDa protein on sequential immunoblots of ammonium sulfate-precipitated filtrate. In addition sera from vaccinated sheep recognized at least eight bacterial cellular antigens on immunoblots of ether-extracted C… Expand
Identification of a novel antigen from Corynebacterium pseudotuberculosis that protects sheep against caseous lymphadenitis
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Results strongly suggest that the 40-kDa antigen plays a major role in immunity to caseous lymphadenitis. Expand
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It is concluded that subcutaneous administration of rHsp60 does not induce effective protection against intrape- ritoneal infection with C. pseudotuberculosis. Expand
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Both humoral and cellular responses kinetics presented a similar pattern of activation/deactivation but necropsy results suggested that the IFN-gamma test would be a very specific marker of CLA status. Expand
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Corynebacterium pseudotuberculosis is the cause of caseous lymphadenitis (CLA) in small ruminants, a chronic granulomatous disease that provokes significant zootechnics losses to ovine and goatExpand
Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice
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This vaccination induced significant anti-hsp60 IgG, IgG1 and IgG2a isotype production, however, immunization with this DNA vaccine did not confer protective immunity. Expand
A Corynebacterium pseudotuberculosis bacterin with muramyl dipeptide induces antibody titers, increases the time of onset, and decreases naturally occurring external abscesses in sheep and goats
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Immunizing effects of live Corynebacterium pseudotuberculosis in goats
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It was concluded that small doses of live C. pseudotuberculosis stimulated an immune response capable of preventing theDevelopment of abscesses in the draining lymph node as well as protected against the development of internal abscesss. Expand
Immunoblot and Ultrastructural Analysis of Antigens Extracted from Dichelobacter Nodosus with Potassium Thiocyanate
  • R. Whittington
  • Biology, Medicine
  • Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
  • 1996
The antigens extracted from Dichelobacter nodosus with potassium thiocyanate were analyzed by western blotting with sera from footrot-free sheep and from sheep infected with D. nodosus to identifyExpand
Reconhecimento de antígenos por anticorpos de caprinos naturalmente infectados ou imunizados contra Corynebacterium pseudotuberculosis
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The analysis of the Western blotting results showed that the antigenic pattern recognized by sera antibodies of immunized animals is similar in crossbred or pure race animals and in naturally infected ones as well. Expand
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TLDR
Protection induced in mice by 300 micrograms of WC was not enhanced by adding any adjuvants and the degree of protection increased with the dose of WC or CW; however, intraperitoneal inoculation with Corynebacterium parvum did not induce resistance to intravenous inoculation. Expand
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