Antigen-specific activation and cytokine-facilitated expansion of naive, human CD8+ T cells

  title={Antigen-specific activation and cytokine-facilitated expansion of naive, human CD8+ T cells},
  author={Matthias W{\"o}lfl and Philip D. Greenberg},
  journal={Nature Protocols},
Antigen-specific priming of human, naive T cells has been difficult to assess. Owing to the low initial frequency in the naive cell pool of specific T cell precursors, such an analysis has been obscured by the requirements for repeated stimulations and prolonged culture time. In this protocol, we describe how to evaluate antigen-specific priming of CD8+ cells 10 d after a single specific stimulation. The assay provides reference conditions, which result in the expansion of a substantial… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 12 times over the past 90 days. VIEW TWEETS
21 Citations
66 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 21 extracted citations


Publications referenced by this paper.
Showing 1-10 of 66 references

CD8 T cell priming in the presence of IFN-α renders CTLs with improved responsiveness to homeostatic cytokines and recall antigens: important traits for adoptive T cell therapy

  • S Hervas-Stubbs
  • J. Immunol
  • 2012

Similar Papers

Loading similar papers…