Antigen-epitope retrieval to facilitate proteomic analysis of formalin-fixed archival brain tissue.

  title={Antigen-epitope retrieval to facilitate proteomic analysis of formalin-fixed archival brain tissue.},
  author={Farid Rahimi and Claire E. Shepherd and Glenda M Halliday and Carolyn L. Geczy and Mark J. Raftery},
  journal={Analytical chemistry},
  volume={78 20},
Formalin is a routine fixative facilitating tissue preservation and histopathology. Proteomic techniques require freshly frozen specimens, which are often difficult to procure, and methods facilitating proteomic analysis of archival formalin-fixed brain tissue are lacking. We employed antigen-epitope-retrieval principles to facilitate proteomic analysis of brain tissue that had been fixed and stored in formalin for 3-7 years. Twenty-micrometer-thick cryopreserved OCT-embedded sections from… CONTINUE READING
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