Antigen-epitope retrieval to facilitate proteomic analysis of formalin-fixed archival brain tissue.

@article{Rahimi2006AntigenepitopeRT,
  title={Antigen-epitope retrieval to facilitate proteomic analysis of formalin-fixed archival brain tissue.},
  author={Farid Rahimi and Claire E. Shepherd and Glenda M Halliday and Carolyn L. Geczy and Mark J. Raftery},
  journal={Analytical chemistry},
  year={2006},
  volume={78 20},
  pages={
          7216-21
        }
}
Formalin is a routine fixative facilitating tissue preservation and histopathology. Proteomic techniques require freshly frozen specimens, which are often difficult to procure, and methods facilitating proteomic analysis of archival formalin-fixed brain tissue are lacking. We employed antigen-epitope-retrieval principles to facilitate proteomic analysis of brain tissue that had been fixed and stored in formalin for 3-7 years. Twenty-micrometer-thick cryopreserved OCT-embedded sections from… CONTINUE READING
Highly Cited
This paper has 23 citations. REVIEW CITATIONS

Citations

Publications citing this paper.
Showing 1-10 of 14 extracted citations

MALDI MS Imaging zur Untersuchung von synovialem Gewebe

View 9 Excerpts
Method Support
Highly Influenced

Antigen retrieval immunohistochemistry: review and future prospects in research and diagnosis over two decades.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society • 2011
View 2 Excerpts
Method Support