Antigen‐specific electrophoretic cell separation for immunological investigations

@article{Hansen1989AntigenspecificEC,
  title={Antigen‐specific electrophoretic cell separation for immunological investigations},
  author={Ernil Hansen and Thomas P. U. Wustrow and Kurt Hannig},
  journal={ELECTROPHORESIS},
  year={1989},
  volume={10}
}
Preincubation of human blood lymphocytes with cell surface antigen specific antibodies under non‐capping conditions reduces the electrophoretic mobility of the corresponding lymphocyte subpopulation. Antigen‐positive and antigen‐negative cells can be separated by free flow electrophoresis with high yield, purity and viability. The use of fluorescence‐labelled second antibodies augments the induced decrease in net surface charge density, and allows rapid detection of antigen‐positive cells in… 
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