Antibody purification: ion-exchange chromatography.

  title={Antibody purification: ion-exchange chromatography.},
  author={A. C. Grodzki and Elsa Berenstein},
  journal={Methods in molecular biology},
  • A. C. Grodzki, Elsa Berenstein
  • Published 2010
  • Chemistry, Medicine
  • Methods in molecular biology
  • Ion exchange chromatography techniques are the focus of this chapter and they showcase the power of this method for the purification of proteins and monoclonal antibodies. The technique is powerful and can separate biomolecules that have minor differences in their net charge, e.g., two protein molecules differing by a single charged amino acid. Given the amphoteric character of proteins the pH of the solution is important in the determination of the type of ion exchanger used. Immunoglobulins… CONTINUE READING
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