Antibody humanization using monovalent phage display.

@article{Baca1997AntibodyHU,
  title={Antibody humanization using monovalent phage display.},
  author={Manuel Baca and Leonard G. Presta and S. J. O'connor and James A. Wells},
  journal={The Journal of biological chemistry},
  year={1997},
  volume={272 16},
  pages={10678-84}
}
Antibody humanization often requires the replacement of key residues in the framework regions with corresponding residues from the parent non-human antibody. These changes are in addition to grafting of the antigen-binding loops. Although guided by molecular modeling, assessment of which framework changes are beneficial to antigen binding usually requires the analysis of many different antibody mutants. Here we describe a phage display method for optimizing the framework of humanized antibodies… CONTINUE READING

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