Antibody humanization using monovalent phage display.

  title={Antibody humanization using monovalent phage display.},
  author={Manuel Baca and Leonard G. Presta and S. J. O'connor and James A. Wells},
  journal={The Journal of biological chemistry},
  volume={272 16},
Antibody humanization often requires the replacement of key residues in the framework regions with corresponding residues from the parent non-human antibody. These changes are in addition to grafting of the antigen-binding loops. Although guided by molecular modeling, assessment of which framework changes are beneficial to antigen binding usually requires the analysis of many different antibody mutants. Here we describe a phage display method for optimizing the framework of humanized antibodies… CONTINUE READING

From This Paper

Topics from this paper.
31 Citations
0 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 31 extracted citations

Similar Papers

Loading similar papers…