Antibody domain mutants demonstrate autonomy of the antigen binding site.

@article{Simon1990AntibodyDM,
  title={Antibody domain mutants demonstrate autonomy of the antigen binding site.},
  author={T. Simon and K. Rajewsky},
  journal={The EMBO journal},
  year={1990},
  volume={9 4},
  pages={1051-6}
}
We have constructed derivatives of a lambda I light chain-bearing anti-(4-hydroxy,3-nitrophenyl)acetyl (NP) antibody which have the V regions exchanged between heavy chains and light chains of the kappa or lambda I type. These antibodies are assembled and secreted normally, and bind haptenic and macromolecular ligands like the wild-type; similar results are obtained for monovalent heterodimers of VHCL and lambda I light chains. The observed independence of the binding site from the constant… CONTINUE READING