Antibody diversity in trouts obtained by gynogenesis or self-fertilization. Comparative analysis of the heavy chain spectrotypes.

@article{Desvaux1987AntibodyDI,
  title={Antibody diversity in trouts obtained by gynogenesis or self-fertilization. Comparative analysis of the heavy chain spectrotypes.},
  author={F X Desvaux and M Cossarini-Dunier and S Chilmonzcyk and Jacques Charlemagne},
  journal={Developmental and comparative immunology},
  year={1987},
  volume={11 3},
  pages={577-84}
}
Conventionnal (C) trouts and trouts obtained by gynogenesis (G) or self-fertilization (SF) were immunized with DNP-KLH and anti-DNP antibodies were individually purified by affinity chromatography. The isolated IgM-like antibodies were separated by an iso-electrofocusing technique in reducing conditions and electroblotted onto nitrocellulose. The transfers… CONTINUE READING