Antibody binding to the juxtamembrane region of the insulin receptor alters receptor affinity.

The effect of three antibodies that interact with distinct regions of the insulin receptor (the alpha subunit (83-7), the juxtamembrane region near tyrosine 960 (960) or the carboxy terminal region of the beta subunit (CT-1) on insulin binding was examined. Detergent-solubilized insulin receptors from IM-9 cells immobilized on Sepharose beads by 960… (More)