Antibody-based nanoprobe for measurement of a fluorescent analyte in a single cell

@article{VoDinh2000AntibodybasedNF,
  title={Antibody-based nanoprobe for measurement of a fluorescent analyte in a single cell},
  author={T. Vo-Dinh and Jean-Pierre Alarie and Brian M. Cullum and G. D. Griffin},
  journal={Nature Biotechnology},
  year={2000},
  volume={18},
  pages={764-767}
}
We report here the application of an antibody-based nanoprobe for in situ measurements of a single cell. The nanoprobe employs antibody-based receptors targeted to a fluorescent analyte, benzopyrene tetrol (BPT), a metabolite of the carcinogen benzo[a]pyrene (BaP) and of the BaP–DNA adduct. Detection of BPT is of great biomedical interest, since this species can serve as a biomarker for monitoring DNA damage due to BaP exposure and for possible precancer diagnosis. The measurements were… CONTINUE READING