Antibody-based enrichment of peptides on magnetic beads for mass-spectrometry-based quantification of serum biomarkers.

@article{Whiteaker2007AntibodybasedEO,
  title={Antibody-based enrichment of peptides on magnetic beads for mass-spectrometry-based quantification of serum biomarkers.},
  author={Jeffrey R Whiteaker and Lei Zhao and Heidi Zhang and Li-Chia Feng and Brian D Piening and Leigh A Anderson and Amanda G. Paulovich},
  journal={Analytical biochemistry},
  year={2007},
  volume={362 1},
  pages={44-54}
}
A major bottleneck for validation of new clinical diagnostics is the development of highly sensitive and specific assays for quantifying proteins. We previously described a method, stable isotope standards with capture by antipeptide antibodies, wherein a specific tryptic peptide is selected as a stoichiometric representative of the protein from which it is cleaved, is enriched from biological samples using immobilized antibodies, and is quantitated using mass spectrometry against a spiked… CONTINUE READING