Antibody-based Proteomics for Human Tissue Profiling
@article{Uhln2005AntibodybasedPF, title={Antibody-based Proteomics for Human Tissue Profiling}, author={Mathias Uhl{\'e}n and Fredrik Pont{\'e}n}, journal={Molecular \& Cellular Proteomics}, year={2005}, volume={4}, pages={384 - 393} }
Here, we describe the use of antibody-based proteomics involving the generation of protein-specific antibodies to functionally explore the human proteome. The antibodies can be used for analysis of corresponding proteins in a wide range of assay platforms, including i) immunohistochemistry for detailed tissue profiling, ii) specific affinity reagents for various functional protein assays, and iii) capture (“pull-down”) reagents for purification of specific proteins and their associated…
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