Antibody-based Proteomics for Human Tissue Profiling

  title={Antibody-based Proteomics for Human Tissue Profiling},
  author={Mathias Uhl{\'e}n and Fredrik Pont{\'e}n},
  journal={Molecular \& Cellular Proteomics},
  pages={384 - 393}
Here, we describe the use of antibody-based proteomics involving the generation of protein-specific antibodies to functionally explore the human proteome. The antibodies can be used for analysis of corresponding proteins in a wide range of assay platforms, including i) immunohistochemistry for detailed tissue profiling, ii) specific affinity reagents for various functional protein assays, and iii) capture (“pull-down”) reagents for purification of specific proteins and their associated… 

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