Antibodies to the ventral disc protein delta-giardin prevent in vitro binding of Giardia lamblia trophozoites.

@article{Jenkins2009AntibodiesTT,
  title={Antibodies to the ventral disc protein delta-giardin prevent in vitro binding of Giardia lamblia trophozoites.},
  author={Mark Christopher Jenkins and Celia N O'Brien and Charles W Murphy and Ryan S Schwarz and Katarzyna B. Miska and Benjamin M Rosenthal and James M. Trout},
  journal={The Journal of parasitology},
  year={2009},
  volume={95 4},
  pages={895-9}
}
A cDNA coding for detlaq-giardin was cloned from Giardia lamblia trophozoites to localize the protein and to study its function in mediating surface attachment. Recombinant delta-giardin antigen was expressed in Escherichia coli as a poly-histidine fusion protein and was purified by affinity chromatography for production of antisera to delta-giardin. By immunoblotting analysis, antisera to recombinant delta-giardin antigen recognized a 31-kDa protein on G. lamblia trophozoites. Anti-recombinant… CONTINUE READING
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Contri - bution of microscopy to a better knowledge of the biology of Giardia lamblia

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