Antibodies to gap-junctional protein selectively disrupt junctional communication in the early amphibian embryo

@article{Warner1984AntibodiesTG,
  title={Antibodies to gap-junctional protein selectively disrupt junctional communication in the early amphibian embryo},
  author={ANNE E. Warner and Sarah Guthrie and Norton B. Gilula},
  journal={Nature},
  year={1984},
  volume={311},
  pages={127-131}
}
Antibodies to the major protein of rat liver gap junctions, molecular weight 27,000 (27K), have been microinjected into one identified cell of 8-cell stage Xenopus embryos. This treatment selectively disrupts both dye transfer and electrical coupling between the progeny cells. These results provide evidence that the 27K protein is an integral component of the cell-to-cell junctional channel. The disruption of junctional communication at early stages results in specific developmental defects… 

The use of antibodies to gap junction protein to explore the role of gap junctional communication during development.

  • A. Warner
  • Biology
    Ciba Foundation symposium
  • 1987
TLDR
The role of communication through gap junctions in the early amphibian and mouse embryos is explored and the possible significance of these findings in relation to development in general and to the organization of the first transporting epithelia to appear during development is discussed.

Effect of antibodies against gap junction protein on gap junction formation in early amphibian embryogenesis

TLDR
It was concluded that the normal assembly of intramembranous particles to form gap junctions was attenuated by microinjection of the antibodies.

Gap junctional communication and development

Patterns of junctional communication in the early amphibian embryo

TLDR
Cell–cell transfer of the fluorescent dye, Lucifer yellow6 (molecular weight (Mr) 450) was re-examined in the early Xenopus laevis embryo by injecting the dye into identified cells, as the position of the injected cell within the embryo may be important.

The role of gap junctions in amphibian development.

  • A. Warner
  • Biology
    Journal of embryology and experimental morphology
  • 1985
TLDR
Experiments on the pattern of junctional communication at early stages of development of the amphibian embryo are described and how anti-gap junction antibodies are being used to determine when and where communication through gap junctions may play an important role during development are illustrated.

Selective disruption of gap junctional communication interferes with a patterning process in hydra.

TLDR
Antibodies to the major rat liver gap junction protein recognize a gap junction antigen in hydra and are effective in eliminating junctional communication between hydra cells, suggesting that cell-cell communication via gap junctions is important in this defined tissue patterning process.

Manipulation of gap junctional communication during compaction of the mouse early embryo.

  • H. Goodall
  • Biology
    Journal of embryology and experimental morphology
  • 1986
TLDR
Neither LCM nor anti-EC could reverse cell coupling once it had become established despite their complete reversal of cell flattening, suggesting that the onset of junctional coupling is not dependent upon extensive cell apposition and that the requirement for extracellular Ca2+ resides at a level other than that of cell adhesion.

Gap junctions and cell interactions during development

...

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