Antibodies targeting extractable nuclear antigens: historical development and current knowledge

  title={Antibodies targeting extractable nuclear antigens: historical development and current knowledge},
  author={Joerg Wenzel and Rainer Gerdsen and Manfred Uerlich and Ralf Bauer and Thomas Bieber and Ingrid B. Boehm},
  journal={British Journal of Dermatology},
The extractable nuclear antigens (ENA) are a heterogeneous group of ribonucleoproteins and non-histone proteins with different functions in nuclear metabolism. The detection of antibodies targeting ENA is a well-established tool in the diagnosis of autoimmune diseases such as lupus erythematosus (LE), progressive systemic sclerosis (PSS), polymyositis (PM), dermatomyositis (DM), mixed connective tissue disease (MCTD), SjoÈgren's syndrome (SS) and overlap syndromes. Descriptions of precipitin… 
Prognostic value of auto-antibodies in the serum of Omani patients with gastric cancer.
Auto-antibodies are more prevalent in the serum of patients with gastric cancer compared to healthy controls, and some may prove to be important markers of prognostic values in patients with Gastric cancer.
Current Concepts and Future Directions for the Assessment of Autoantibodies to Cellular Antigens Referred to as Anti-Nuclear Antibodies
A comprehensive overview of the current status on ANA testing including automated IIF reading systems and solid phase assays is provided and an approach to interpretation of results is suggested and meeting the problems of assay standardization and other persistent challenges are discussed.
Immunological Tests in Primary Sjögren Syndrome
Patients with primary Sjogren syndrome (SS) produce a wide variety of autoantibodies directed at specific nuclear or cytoplasmic antigens. In some cases, the target antigen is present within specific
Primary Sjögren’s Syndrome and Autoantibodies
This chapter outlines the autoantibodies found in Sjögren’s syndrome and discusses their importance in clinical practice.
Clinical and Pathological Roles of Ro/SSA Autoantibody System
The milestones of the anti-Ro/SSA autoantibody system are summarized and new insights are provided into the association between theAutoantibodies and the pathogenesis of autoimmune diseases.
Immunoglobulin-like transcripts 6 (ILT6) polymorphism influences the anti-Ro60/52 autoantibody status in south Indian SLE patients
ILT6 deletion polymorphism does not appear to be a lupus susceptibility gene in South Indian Tamils, but may behave as a genetic modifier of autoantibody phenotype by influencing the production of anti-Ro60 andAnti-Ro52 autoantIBodies and thus indirectly contribute to autoimmune responses in SLE.
Cellular and molecular factors in the pathogenesis of systemic autoimmunity and comorbidities
The antiinflammatory and anti-proliferative properties of TRIM21/Ro52, one of the targets of antiRo, are investigated, and genetic variants of the CXCR5 gene locus are connected to expression patterns and cell compartmentalization in SS, demonstrating a role of the autoantigen TRim21 in B cell homeostasis and function.
Evaluation of a novel latex enhanced turbidimetric immunoassay for detecting autoantibody against extractable nuclear antigens
With the pronounced advantages of automatic and rapid detection, and high universality, LETIA can meet the requirements for quantitative detection of anti-ENAs.
Immunopathology of vesicular cutaneous lupus erythematosus in the rough collie and Shetland sheepdog: a canine homologue of subacute cutaneous lupus erythematosus in humans.
Results of these studies provide evidence supporting the hypothesis that canine VCLE is an immunological homologue of subacute cutaneous lupus erythematosus in humans.
An Observational Cross-Sectional Study of Varied Clinical Manifestations of Connective Tissue Disorders and their Association with Antinuclear Antibodies in a Tertiary Care Center
Cutaneous manifestations of CTD can be an early predictor in giving a clue to impending systemic manifestations, thus helping in the multidisciplinary management.


Identification of a nuclear protein (Scl-70) as a unique target of human antinuclear antibodies in scleroderma.
A protein antigenic to antibodies from scleroderma patients was isolated from rat liver nuclei using a combination of biochemical and immunologic methods and the result indicated that these antibodies are effectively monospecific for Scl-70.
Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies in sera from patients with polymyositis-scleroderma overlap.
Preliminary results of clinical analysis indicated that antibody to the Ku antigen might become a useful marker for a group of patients with clinical characteristics of both polymyositis and scleroderma with a good prognosis.
Antinuclear antibody with distinct specificity for polymyositis.
Fractionation of PM-1-positive serum by 30% ammonium sulphate and Sephadex G-200 chromatography revealed that the factor producing thePM-1 precipitin reaction was in a serum fraction which showed only IgG by immunoelectrphoresis against anti-whole human serum.
Antibodies to Mi-1 in SLE: relationship to other precipitins and reaction with bovine immunoglobulin.
Anti-Mi-1 cross-reacts directly with BIgG but differs from ordinary rheumatoid factor in its frequent reaction with Fab2 fragments and restricted species specificity, which means it can be found in patients with diseases other than DM, including SLE, most often in Patients with antibodies to nRNP.
Heterogeneity of precipitating antibodies in polymyositis and dermatomyositis. Characterization of the Jo-1 antibody system.
A 600-fold purification of the most frequently appearing system designated Jo-1 was achieved and its clinical specificity for patients with PM syndromes was established.
Differentiation and characterization of autoantibodies and their antigens in Sjögren's syndrome.
These studies show that precipitins SS-A and SS-B are present in high frequency in sera of patients with SS without associated rheuamtoid arthritis (RA) and are absent or present in low frequency in many other connective tissue diseases.
Humoral immunity in polymyositis/dermatomyositis.
  • I. Targoff
  • Biology, Medicine
    The Journal of investigative dermatology
  • 1993
Understanding of the reasons for production of these antibodies should provide insight into the etiology and pathogenesis of PM and DM, and several autoantibodies, including anti-PM-Scl, anti-Ku, and anti-U1 and U2 RNP, have been associated with scleroderma-PM overlap.
Characterization of a soluble nuclear ribonucleoprotein antigen reactive with SLE sera.
A soluble nuclear ribonucleoprotein antigen reactive with SLE sera in precipitin and complement fixation reactions is described. The antigen was shown to be quite labile and was not recovered from
Clinical and serologic associations of the antiribosomal P protein antibody.
Western blotting was the most sensitive and specific method for the detection of anti-P antibodies; counter-immunoelectrophoresis and cytoplasmic indirect immunofluorescence were positive in only 47% and 65% of theAnti-P-positive patients, respectively.
The association between Mi-2 antibodies and dermatomyositis.
Anti-Mi-2 appears to be closely linked to DM, and is the first specific serologic marker for this form of myositis, and was significantly more frequent in DM than in PM.