Antibodies in haystacks: how selection strategy influences the outcome of selection from molecular diversity libraries.

@article{Lou2001AntibodiesIH,
  title={Antibodies in haystacks: how selection strategy influences the outcome of selection from molecular diversity libraries.},
  author={Jianlong Lou and Roberto Marzari and Vittorio Verzillo and Fulvio Ferrero and Dohyun Pak and Maw Sheng and C Yang and Daniele Sblattero and A. S. C. Bradbury},
  journal={Journal of immunological methods},
  year={2001},
  volume={253 1-2},
  pages={233-42}
}
Antibodies against most antigens can be isolated from high quality phage antibody libraries. However, not all antibodies binding a particular antigen are necessarily found when standard selections are performed. Here we investigate the effect of two different selection strategies on the isolation of antibodies against a number of different antigens, and find that these different strategies tend to select different antibodies, with little overlap between them. This indicates that the full… CONTINUE READING

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