Antibodies as Thermolabile Switches: High Temperature Triggering for the Polymerase Chain Reaction

@article{Sharkey1994AntibodiesAT,
  title={Antibodies as Thermolabile Switches: High Temperature Triggering for the Polymerase Chain Reaction},
  author={David J. Sharkey and Edward R. Scalice and Kenneth George Christy and Susan Melissa Atwood and John L. Daiss},
  journal={Bio/Technology},
  year={1994},
  volume={12},
  pages={506-509}
}
We demonstrate the utility of antibodies to the DNA polymerase from Thermus aquaticus (TaqPol) as thermolabile inhibitors of TaqPol activity. One of the limitations of the polymerase chain reaction (PCR) is the co-amplification of non-specific products caused by TaqPol activity on low stringency templates present in the initial cycle of PCR. We have used anti-TaqPol antibodies as thermolabile switches that inhibit TaqPol activity at low temperatures (20–40°C) and release fully active TaqPol… Expand
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