Antibodies against the C-terminal peptide of rabbit oviductin inhibit mouse early embryo development to pass 2-cell stage

  title={Antibodies against the C-terminal peptide of rabbit oviductin inhibit mouse early embryo development to pass 2-cell stage},
  author={P. Yong and Zheng Gu and Jin Ping Luo and Jun Ru Wang and J. Y. Tso},
  journal={Cell Research},
  • P. Yong, Zheng Gu, +2 authors J. Y. Tso
  • Published 2002
  • Medicine, Biology
  • Cell Research
  • ABSTRACTA full-length rabbit oviductin cDNA(1909bp) was cloned. It consists of a 5′-UTR of 52bp, an open reading frame (ORF) of 1374bp and a 3′ -UTR of 483bp and has more than 80% homology with that of other mammal oviductins. N-terminal peptide (NTP) (384 residues) and C-terminal peptide (CTP) (73 residues) of deduced protein precursor has about 80% and 50% identity with that of other mammals respectively. Fusion proteins GST-NTP 368(1R-368N)and GST-CTP73 (369F-441A) were expressed and… CONTINUE READING
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