Antibodies against insulin measured by electrochemiluminescence predicts insulitis severity and disease onset in non-obese diabetic mice and can distinguish human type 1 diabetes status

@inproceedings{Lo2011AntibodiesAI,
  title={Antibodies against insulin measured by electrochemiluminescence predicts insulitis severity and disease onset in non-obese diabetic mice and can distinguish human type 1 diabetes status},
  author={Bernice Lo and Austin DE Swafford and Kimberly A. Shafer-Weaver and Lawrence F Jerome and Luba Rakhlin and Douglas R Mathern and Conor A Callahan and Ping Jiang and L J Davison and Helen E. Stevens and Carrie L Lucas and Jill White and Reid W. von Borstel and John A. Todd and Michael J Lenardo},
  booktitle={Journal of Translational Medicine},
  year={2011}
}
The detection of insulin autoantibodies (IAA) aids in the prediction of autoimmune diabetes development. However, the long-standing, gold standard 125I-insulin radiobinding assay (RBA) has low reproducibility between laboratories, long sample processing times and requires the use of newly synthesized radiolabeled insulin for each set of assays. Therefore, a rapid, non-radioactive, and reproducible assay is highly desirable. We have developed electrochemiluminescence (ECL)-based assays that… CONTINUE READING