Antibiotic susceptibility testing by a standardized single disk method.

  title={Antibiotic susceptibility testing by a standardized single disk method.},
  author={A W Bauer and William M. M. Kirby and John C. Sherris and Marvin Turck},
  journal={American journal of clinical pathology},
  volume={45 4},
In the words of the authors, the paper by A. W. Bauer et al., from the University of Washington in Seattle, on a standardized single-disk method for antibiotic susceptibility testing “. . . consolidate(s) and update(s) previous descriptions of the method and provide(s) a concise outline for its performance and interpretation.” Clinical microbiologists were relieved that finally a disk diffusion method had been standardized, could be used with ease, and provided reliable results as compared with… 

An improved single-disk method for testing the antibiotic susceptibility of rapidly-growing pathogens.

Because the inhibitory zone edges were better defined on plates inoculated with the agar-overlay method, there was a slightly better correlation between zone diameters and minimal inhibitory concentrations, especially with bacteriostatic drugs.

Setting interpretive breakpoints for antimicrobial susceptibility testing using disk diffusion.

Simple inoculum standardizing system for antimicrobial disk susceptibility tests

The Inocupac system is a valid alternative method for inoculating disk susceptibility tests and varied from 90 to 99% with different antimicrobial agents, and repeated tests with the Bauer-Kirby method demonstrated the same degree of interpretive agreement.

A study of antibiotic sensitivity testing with proposals for simple uniform methods

Indications and methods for tests in primary culture, and proposals are made for eliminating superfluous or inappropriate tests, thus limiting the number of antibiotics or other drugs with which tests need initially be done.

Comparison of direct and standard antimicrobial disk susceptibility testing for bacteria isolated from blood

Direct susceptibility testing of positive blood cultures that were pure by gram-stained smear provided reliable results 24 to 36 h earlier than conventional procedures; therefore, this procedure is recommended to guide early antimicrobial therapy in patients with bacterial sepsis.

Determination of antimicrobial MIC by paper diffusion method.

  • M. Shafi
  • Biology
    Journal of clinical pathology
  • 1975
A simple method of incorporating the antimicrobial drug in agar is described, an adaptation of the Rolinson and Russell technique which allows the determination of minimum inhibitory concentrations (MIC) of antimicrobial drugs for a large number of organisms.

Evaluation of the MS-2 automated antimicrobial susceptibility testing system: Report of a European collaborative study

Tests on independently isolated organisms showed the MS-2 to be in essential agreement with conventional methods in 94.8% of tests and compared at least as well with the reference values as did either of the manual methods.

Interpretive Criteria for the Agar Diffusion Susceptibility Test with Ofloxacin

It was found that the correlation of minimal inhibitory concentrations (MICs) and inhibition zones produced by ofloxacin discs becomes poorer with increasing disc loads and when the Kirby-Bauer method is used.

History and development of antimicrobial susceptibility testing methodology.

  • P. Wheat
  • Medicine
    The Journal of antimicrobial chemotherapy
  • 2001
The history and development of some methods still in common use for routine AST, e.g. disc diffusion and agar dilution are traced, to increase the speed and reliability of resistance testing, the use of a genotypic approach has been advocated.

Erythromycin-Resistant Group A Streptococci-Reply

In Reply .—The results obtained by Dr Garber demonstrate very well how microorganisms of a single species are not static, but vary in their properties from place to place and from time to time. His