Antibiotic optimization via in vitro glycorandomization

@article{Fu2003AntibioticOV,
  title={Antibiotic optimization via in vitro glycorandomization},
  author={Xun Fu and Christoph Albermann and Jiqing Jiang and Jianchun Liao and Changsheng Zhang and Jon S. Thorson},
  journal={Nature Biotechnology},
  year={2003},
  volume={21},
  pages={1467-1469}
}
In nature, the attachment of sugars to small molecules is often used to mediate targeting, mechanism of action and/or pharmacology. As an alternative to pathway engineering or total synthesis, we report a useful method, in vitro glycorandomization (IVG), to diversify the glycosylation patterns of complex natural products. We have used flexible glycosyltransferases on nucleotide diphosphosugar (NDP-sugar) libraries to generate glycorandomized natural products and then applied chemoselective… 

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