Antibiotic-induced release of endotoxin from bacteria in vitro.

@article{Crosby1994AntibioticinducedRO,
  title={Antibiotic-induced release of endotoxin from bacteria in vitro.},
  author={Heather A. Crosby and Julian Bion and Charles W. Penn and Tom S.J. Elliott},
  journal={Journal of medical microbiology},
  year={1994},
  volume={40 1},
  pages={
          23-30
        }
}
The ability of cefotaxime, ciprofloxacin, piperacillin and tobramycin to cause release of endotoxin was examined in vitro with cultures of Enterobacter cloacae and Escherichia coli. Endotoxin was measured by a quantitative limulus amoebocyte lysate assay and its presence was confirmed by silver staining of the lipopolysaccharide moiety following SDS-PAGE. The morphology of the bacteria during antibiotic exposure was examined by scanning electronmicroscopy. Cefotaxime, ciprofloxacin and… 
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Influence of In-Vivo Endotoxin Liberation on Anti-Anaerobic Antimicrobial Efficacy
TLDR
It is conceivable that if the gastrointestinal tract is the source of the endotoxin in patients with systemic inflammatory response syndrome, then the obligate anaerobes like Bacteroides and Fusobacterium species, which are members of the gut flora, may play a major role in the unfavorable outcome of antibiotic therapy in some of these infections.
Influence of Five Antianaerobic Antibiotics on Endotoxin Liberation by Gram-Negative Anaerobes
TLDR
Seven strains of the Bacteroides fragilis group were studied for their ability to liberate endotoxin upon exposure to five anti-anaerobic antibiotics, to varying degrees, and all five antibiotics had the capacity to induce endotoxin liberation by Gram-negative anaerobic bacteria.
Relevance of antimicrobial agent-induced endotoxin release from in vitro cultured Escherichia coli and in vivo experimental infection with Gram-negative bacilli.
Lincomycin-induced endotoxin release in Escherichia coli sepsis: evidence for release in vitro and in vivo.
  • T. Horii, T. Kimura, M. Nadai, M. Kobayashi
  • Medicine, Biology
    International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
  • 2000
Endotoxin release due to ciprofloxacin measured by three different methods.
TLDR
Limulus LPS determinations correlated more precisely with LPS bioactivity than did ELISA values, an observation which underlines the crucial role of LPS determination methods in studies of antibiotic-induced LPS release.
Variation in the Propensity to Release Endotoxin after Cefuroxime Exposure in Different Gram-negative Bacteria: Uniform and Dose-dependent Reduction by the Addition of Tobramycin
An aminoglycoside in combination with a β-lactam antibiotic is often recommended for the treatment of severe infections. The aim of the present study was to study whether cefuroxime-induced endotoxin…
Antimicrobial-induced endotoxin and cytokine activity in an in vitro model of septicemia in foals.
TLDR
Aminoglycosides appear less likely to induce endotoxemia and TNF-alpha synthesis during bactericidal treatment of E. coli septicemia, compared with beta-lactam antimicrobials.
In vivo TNF induction by culture supernatants of antibiotic-treated Escherichia coli 07:K1. Role of antibiotic class and concentration.
Antibiotic-induced Bacterial Toxin Release – Inhibition by Protein Synthesis Inhibitors
TLDR
The propensity to liberate endotoxin is higher after the second dose of cefuroxime than after the first, resulting in a higher release of endotoxin than expected from bacterial count, which can be reduced by the addition of tobramycin.
β-lactam antibiotic-induced release of lipoteichoic acid from Staphylococcus aureus leads to activation of neutrophil granulocytes
TLDR
The findings suggest that, via the activation of PMN, antibiotic-induced LTA release from S. aureus leads to enhanced antimicrobial activity of the innate immune defense mechanisms.
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References

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