Antiapoptotic effect both in vivo and in vitro of A20 gene when transfected into rat hippocampal neurons


AbstractAim:To evaluate the antiapoptotic effect of the A20 gene in primary hippocampal neurons both in vivo and in vitro.Methods:Primary hippocampal neurons in embryonic day 18 (E18) rats were transfected with the A20 gene by using the new Nucleofector electroporation transfection method. We then examined, whether A20 -neurons possessed anti-apoptotic abilities after TNF-α stimulation in vitro. A20-neurons and pcDNA3 -neurons were transplanted into the penumbra of the brains of rats that had been subjected to 90-min of ischemia induced by left middle cerebral artery occlusion (MCAO).Results:A20-neurons resisted TNF-α induced apoptosis in vitro. The apoptosis rate of neurons overexpressing A20 (28.46%±3.87%) was lower than that in neurons transfected with pcDNA3 (53.06%±5.36%). More A20-neurons survived in the penumbra both 3-d and 7-d after transplantation than did sham pcDNA3 neurons.Conclusion:The novel function of A20 may make it a potential targets for the gene therapy for neurological diseases.

DOI: 10.1111/j.1745-7254.2005.00002.x

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@article{Miao2005AntiapoptoticEB, title={Antiapoptotic effect both in vivo and in vitro of A20 gene when transfected into rat hippocampal neurons}, author={Hong-sheng Miao and Lu-yang Yu and Guo-zhen Hui and Li-he Guo}, journal={Acta Pharmacologica Sinica}, year={2005}, volume={26}, pages={33-38} }