Anti-tumor effect of pEgr-IFNγ gene-radiotherapy in B16 melanoma-bearing mice

@article{Wu2004AntitumorEO,
  title={Anti-tumor effect of pEgr-IFN$\gamma$ gene-radiotherapy in B16 melanoma-bearing mice},
  author={Cong-mei Wu and Xiu-yi Li and Tian-hua Huang},
  journal={World Journal of Gastroenterology},
  year={2004},
  volume={10},
  pages={3011-3015}
}
AIM: To construct a pEgr-IFNγ plasmid and to investigate its expression properties of interferon-γ (INF-γ ) induced by irradiation and the effect of gene-radiotherapy on the growth of melanoma. METHODS: A recombined plasmid, pEgr-IFNγ , was constructed and transfected into B16 cell line with lipofectamine. The expression properties of pEgr-IFNγ were investigated by ELISA. Then, a B16 melanoma-bearing model was established in mice, and the plasmid was injected into the tumor tissue. The tumor… Expand
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