Anti-tumor effect of pEgr-IFNγ gene-radiotherapy in B16 melanoma-bearing mice

  title={Anti-tumor effect of pEgr-IFN$\gamma$ gene-radiotherapy in B16 melanoma-bearing mice},
  author={Cong-mei Wu and Xiu-yi Li and Tian-hua Huang},
  journal={World Journal of Gastroenterology},
AIM: To construct a pEgr-IFNγ plasmid and to investigate its expression properties of interferon-γ (INF-γ ) induced by irradiation and the effect of gene-radiotherapy on the growth of melanoma. METHODS: A recombined plasmid, pEgr-IFNγ , was constructed and transfected into B16 cell line with lipofectamine. The expression properties of pEgr-IFNγ were investigated by ELISA. Then, a B16 melanoma-bearing model was established in mice, and the plasmid was injected into the tumor tissue. The tumor… Expand
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The treatment of established intracranial tumors by in situ retroviral IFN-γ transfer
Investigation of the efficacy of treating established rat C6 glioma by in situ retroviral delivery of IFN-γ cDNA in immunocompetent Wistar rats found that this therapeutic strategy may provide an effective method of eradicating established intracranial tumors. Expand
Combined effects of radiotherapy and angiostatin gene therapy in glioma tumor model.
  • F. Griscelli, H. Li, +8 authors P. Yeh
  • Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2000
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Gene therapy targeted by radiation preferentially radiosensitizes tumor cells.
The combination of gene therapy and radiation therapy enhances tumor cures without increasing normal tissue toxicity and is a new paradigm for cancer treatment. Expand
The anti-tumor activity of anti-CTLA-4 is mediated through its induction of IFNγ
It is demonstrated that one mechanism for the anti-tumor effects of anti-CTLA-4 relates to its ability to augment IFNγ production, resulting in an increased expression of class I on the tumor, enhanced apoptosis, and a decrease in blood vessel growth. Expand
Transduction of human renal carcinoma cells with human gamma-interferon gene via retroviral vector.
It is confirmed that retroviral vector transduction of the human gamma-ifN gene into renal carcinoma cells is feasible and associated with persistent production of gamma-IFN and increased expression of HLA class I and II molecules, and these effects are retained after irradiation and cryopreservation. Expand
Induction of the suicide HSV-TK gene by activation of the Egr-1 promoter with radioisotopes.
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Systemic IFN-β gene therapy results in long-term survival in mice with established colorectal liver metastases
HIFN-β gene therapy not only resulted in improved survival and long-term cure in a micrometastatic model, but provided similar benefits in a clinically relevant gross disease model. Expand
Antitumor interaction of short-course endostatin and ionizing radiation.
Results from the clonogenic and apoptosis assays support the hypothesis that the endothelial compartment is the target for the endostatin/IR interaction and suggest additive antitumor effects in both human and murine tumors. Expand
Antiviral activities of individual murine IFN-alpha subtypes in vivo: intramuscular injection of IFN expression constructs reduces cytomegalovirus replication.
Evidence is provided indicating that there are in vivo differences in the antiviral efficacy of the IFN-alpha subtypes, suggesting that the protection observed is due to host cell-mediated IFN signaling. Expand