Anti-sense expression of putrescine N-methyltransferase confirms defensive role of nicotine in Nicotiana sylvestris against Manduca sexta

@article{Voelckel2001AntisenseEO,
  title={Anti-sense expression of putrescine N-methyltransferase confirms defensive role of nicotine in Nicotiana sylvestris against Manduca sexta},
  author={Claudia Voelckel and Tamara Kr{\"u}gel and Klaus Gase and Nadja Heidrich and Nicole M. van Dam and Robert Winz and Ian T. Baldwin},
  journal={CHEMOECOLOGY},
  year={2001},
  volume={11},
  pages={121-126}
}
Several lines of evidence support the defensive function of nicotine production in the Nicotiana genus against a range of herbivores, but the evidence is largely correlative. To suppress nicotine production in planta and to test its defensive function, we expressed DNA of putrescine N-methyl transferase in an anti-sense orientation (AS-PMT) in N. sylvestris and fed leaf material from two lines of transformed and wild type plants to Manduca sexta larvae. Larvae consumed more leaf area and gained… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 23 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 22 references

Extraction of total cellular DNA from plants, algae and fungi

  • SO Rogers, AJ Bendich
  • Plant Molec Biol Man
  • 1994
Highly Influential
1 Excerpt

Similar Papers

Loading similar papers…