• DOI:10.3839/JABC.2014.036
• Corpus ID: 101983574

Anti-inflammatory Activity of Sargassum micracanthum Water Extract

@article{Jeong2014AntiinflammatoryAO,
  title={Anti-inflammatory Activity of Sargassum micracanthum Water Extract},
  author={Da-Hyun Jeong and Bo-Kyeong Kang and Koth-Bong-Woo-Ri Kim and Min-Ji Kim and Dong Hyun Ahn},
  journal={Journal of Applied Biological Chemistry},
  year={2014},
  volume={57},
  pages={227-234}
}

• Da-Hyun Jeong, Bo-Kyeong Kang, +2 authors D. Ahn
• Published 30 September 2014
• Biology
• Journal of Applied Biological Chemistry

The anti-inflammatory effect of Sargassum micracanthum water extract (SMWE) was investigated using lipopolysaccharide (LPS)-induced inflammatory response in this study. The murine macrophage cell line RAW 264.7 cells were used and MTT assay was performed to measure the cell proliferation ability. The secretion of nitric oxide (NO), tumor necrosis factor- (TNF-), interleukin-6 (IL-6), and IL- was measured in LPS-induced RAW 264.7 cells by ELISA. The expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear transcription factor-kappa B p65 protein was studied by immunoblotting. The Balb/c mice were used for an acute toxicity test, and imprinting control region mice were purchased to evaluate a croton oil-induced ear edema. As a result, there was no cytotoxicity in the macrophage proliferation treated with SMWE compared to the control. NO levels decreased with increasing concentration of SMWE and were inhibited over 50%. Moreover, the secretion of IL-6, TNF-, and IL- was suppressed in a dose-dependent manner, especially, IL- inhibition activity was over 50% at 50 /mL. The formation of ear edema of mice was reduced at the highest dose tested compared to that in the control. Moreover, in acute toxicity test, no moralities occurred in mice administered 5,000 mg/kg body weight of SMWE over 2 weeks observation period. These results suggested that SMWE may have significant effects on inflammatory factors and be potential anti-inflammatory therapeutic materials.