Anti-idiotype monoclonal antibodies against anti-microcystin antibody and their use in enzyme immunoassay.

@article{Tsutsumi1998AntiidiotypeMA,
  title={Anti-idiotype monoclonal antibodies against anti-microcystin antibody and their use in enzyme immunoassay.},
  author={Taiki Tsutsumi and Saburo Nagata and Fumi Yoshida and Yoshio Ueno},
  journal={Toxicon : official journal of the International Society on Toxinology},
  year={1998},
  volume={36 2},
  pages={235-45}
}
Microcystins (MCs), a group of heptapeptide hepatotoxins produced by cyanobacteria, are suspected as tumor-promoter contaminants of environmental water. We have previously developed an enzyme-linked immunosorbent assay (ELISA) for MCs based on an anti-MC MAb (MAb-mc). We describe here the production of anti-idiotype MAbs (MAb-ids) which react with MAb-mc and their use in a new ELISA for MCs. For the production of MAb-id, hybridoma cells were generated from mice immunized with MAb-mc. Two MAbs… CONTINUE READING