Anti-human vascular endothelial growth factor (VEGF) antibody selection for immunohistochemical staining of proliferating blood vessels.

@article{Loos2010AntihumanVE,
  title={Anti-human vascular endothelial growth factor (VEGF) antibody selection for immunohistochemical staining of proliferating blood vessels.},
  author={Chris M. van der Loos and Lorine B. Meijer-Jorna and Marloes E C Broekmans and Hanneke P H M Ploegmakers and Peter Teeling and Onno J de Boer and Allard C. van der Wal},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={2010},
  volume={58 2},
  pages={109-18}
}
Nine commercially available vascular endothelial growth factor (VEGF) antibodies were investigated for their ability to immunostain vascular malformations (VMs) with or without immature capillary proliferation. First, all antibodies were optimized for their performance in IHC, with placenta and colon adenocarcinoma as positive control tissues. Five antibodies were regarded as unfit for VEGF immunostaining based on poor immunostaining criteria. Subsequently, Western blot analysis using VEGF… CONTINUE READING