Anti-Proliferative and Pro-Apoptotic Effects of (-)-Epigallocatechin-3-Gallate Encapsulated in Chitosan Nanoparticles on Human Prostate Carcinoma Cells
@inproceedings{Shabana2014AntiProliferativeAP, title={Anti-Proliferative and Pro-Apoptotic Effects of (-)-Epigallocatechin-3-Gallate Encapsulated in Chitosan Nanoparticles on Human Prostate Carcinoma Cells}, author={Sameh M. Shabana and Imtiaz Ahmad Siddiqui and Naghma and Khan and Vaqar M Adhami and Qateeb Khan and Shaker and Mousa and Ahmed E. E. Hagras and Marwa El-Missiry and Hasan and Mukhtar}, year={2014} }
(-)-epigallocatechin-3-gallate (EGCG), the major polyphenol of green tea, has shown excellent effects in preventing, as well as treating, many cancers including prostate cancer (PCa) in preclinical model systems. However its applicability to humans has met with limited success due to several issues including its limited bioavailability. We employed the use of nanotechnology to improve the outcome of cancer chemoprevention and coined the concept ‘nanochemoprevention’. Recently, we reported the…
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Preparation of Catechin Nanoemulsion from Oolong Tea Leaf Waste and Its Inhibition of Prostate Cancer Cells DU-145 and Tumors in Mice
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Catechin nanoemulsions were more effective than extracts in inhibiting DU-145 cell growth, with the IC50 being 13.52 and 214.6 μg/mL, respectively, after 48 h incubation, and could raise caspase-8, caspasing-9 and caspases-3 activities for DU- 145 cell apoptosis, arresting the cell cycle at S and G2/M phases.
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Oral administration of naturally occurring chitosan-based nanoformulated green tea polyphenol EGCG effectively inhibits prostate cancer cell growth in a xenograft model.
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This study proposes a novel preventive and therapeutic modality for PCa using EGCG that addresses issues related to bioavailability and encapsulating useful bioactive food components for their slow and sustained release.
Introducing nanochemoprevention as a novel approach for cancer control: proof of principle with green tea polyphenol epigallocatechin-3-gallate.
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This study encapsulated green tea polyphenol epigallocatechin-3-gallate (EGCG) in polylactic acid-polyethylene glycol nanoparticles and observed that encapsulated EGCG retains its biological effectiveness with over 10-fold dose advantage for exerting its proapoptotic and angiogenesis inhibitory effects, critically important determinants of chemopreventive effects of E GCG in both in vitro and in vivo systems.
(-)-Epigallocatechin-3-gallate reactivates silenced tumor suppressor genes, Cip1/p21 and p16INK4a, by reducing DNA methylation and increasing histones acetylation in human skin cancer cells.
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This study shows that EGCG treatment decreased global DNA methylation levels in A431 cells in a dose-dependent manner and provides new insight into the epigenetic mechanism of action of E GCG that may contribute to the chemoprevention of skin cancer and may have important implications for epigenetic therapy.
Nanochemoprevention by Bioactive Food Components: A Perspective
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This study determined the efficacy of a well-known chemopreventive agent epigallocatechin-3-gallate encapsulated in polylactic acid (PLA)-polyethylene glycol (PEG) nanoparticles in preclinical settings and observed that nano-EGCG retained its biological efficacy with over 10-fold dose advantage both in cell culture system and in vivo settings in athymic nude mice implanted with human prostate cancer cells.
Progress in Nanotechnology Based Approaches to Enhance the Potential of Chemopreventive Agents
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This review summarizes the most up-to-date knowledge on the studies performed in nanochemoprevention, their proposed use in the clinic and future directions in which this field is heading.
Design of curcumin-loaded PLGA nanoparticles formulation with enhanced cellular uptake, and increased bioactivity in vitro and superior bioavailability in vivo.
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Tea polyphenol (-)-epigallocatechin-3-gallate inhibits DNA methyltransferase and reactivates methylation-silenced genes in cancer cell lines.
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It is reported herein that (-)-epigallocatechin-3-gallate (EGCG), the major polyphenol from green tea, can inhibit DNMT activity and reactivate methylation-silenced genes in cancer cells and the potential use of EGCG for the prevention or reversal of related gene-silencing in the prevention of carcinogenesis is suggested.
Chitosan nanoparticles enhance the intestinal absorption of the green tea catechins (+)-catechin and (-)-epigallocatechin gallate.
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Modulation of signaling pathways in prostate cancer by green tea polyphenols.
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Growth inhibition, cell-cycle dysregulation, and induction of apoptosis by green tea constituent (-)-epigallocatechin-3-gallate in androgen-sensitive and androgen-insensitive human prostate carcinoma cells.
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It is suggested that EGCG negatively modulates PCA cell growth, by affecting mitogenesis as well as inducing apoptosis, in cell-type-specific manner which may be mediated by WAF1/p21-caused G(0)/G(1)-phase cell-cycle arrest, irrespective of the androgen association or p53 status of the cells.