Anti-Argonaute RIP-Chip shows that miRNA transfections alter global patterns of mRNA recruitment to microribonucleoprotein complexes.

@article{Wang2010AntiArgonauteRS,
  title={Anti-Argonaute RIP-Chip shows that miRNA transfections alter global patterns of mRNA recruitment to microribonucleoprotein complexes.},
  author={Wang-Xia Wang and Bernard R. Wilfred and Yanling Hu and Arnold J. Stromberg and Peter T. Nelson},
  journal={RNA},
  year={2010},
  volume={16 2},
  pages={394-404}
}
MicroRNAs (miRNAs) play key roles in gene expression regulation by guiding Argonaute (AGO)-containing microribonucleoprotein (miRNP) effector complexes to target polynucleotides. There are still uncertainties about how miRNAs interact with mRNAs. Here we employed a biochemical approach to isolate AGO-containing miRNPs from human H4 tumor cells by co-immunoprecipitation (co-IP) with a previously described anti-AGO antibody. Co-immunoprecipitated (co-IPed) RNAs were subjected to downstream… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 42 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 51 references

association in polyribosomes in a human neuronal cell line

PT Nelson, M DePlanell-Saguer, +4 authors Z Mourelatos
2007
View 10 Excerpts
Highly Influenced

Argonaute proteins: key players in RNA silencing

Nature Reviews Molecular Cell Biology • 2008
View 4 Excerpts
Highly Influenced

miRNP :

PT Nelson, AG Hatzigeorgiou, Z Mourelatos
mRNA • 2004
View 2 Excerpts
Highly Influenced

MicroRNAs and metazoan macroevolution: insights into canalization, complexity, and the Cambrian explosion.

BioEssays : news and reviews in molecular, cellular and developmental biology • 2009
View 1 Excerpt

Similar Papers

Loading similar papers…