Anthroylouabain: a specific fluorescent probe for the cardiac glycoside receptor of the Na-K ATPase.

@article{Fortes1977AnthroylouabainAS,
  title={Anthroylouabain: a specific fluorescent probe for the cardiac glycoside receptor of the Na-K ATPase.},
  author={P. A. Fortes},
  journal={Biochemistry},
  year={1977},
  volume={16 3},
  pages={
          531-40
        }
}
Anthroylouabain (AO) was synthesized by reaction of anthracene-9-carboxylic chloride with ouabain. Nuclear magnetic resonance spectroscopy of AO suggests that the anthracene is esterfied to the rhamnose in the glycoside. AO inhibits Na-K ATPase from human red cells, eel electroplax and rabbit and dog kidney with a KI less than 1muM. AO bound to rabbit or dog kidney Na-K ATPase shows enhanced fluorescence and characteristic spectral shifts. AO binding requires Mg and is optimum in the presence… Expand
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