Antarctic mixotrophic protist abundances by microscopy and molecular methods.

  title={Antarctic mixotrophic protist abundances by microscopy and molecular methods.},
  author={Rebecca J. Gast and Zaid M. McKie-Krisberg and Scott A. Fay and Julie M. Rose and Robert W Sanders},
  journal={FEMS microbiology ecology},
  volume={89 2},
Protists are traditionally described as either phototrophic or heterotrophic, but studies have indicated that mixotrophic species, organisms that combine both strategies, can have significant impacts on prey populations in marine microbial food webs. While estimates of active mixotroph abundances in environmental samples are determined microscopically by fluorescent particle ingestion, species identification is difficult. We developed SYBR-based qPCR strategies for three Antarctic algal species… CONTINUE READING
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