Antagonists Mo and Cu in a heterometallic cluster present on a novel protein (orange protein) isolated from Desulfovibrio gigas.

@article{Bursakov2004AntagonistsMA,
  title={Antagonists Mo and Cu in a heterometallic cluster present on a novel protein (orange protein) isolated from Desulfovibrio gigas.},
  author={Sergey A. Bursakov and Olga Yu Gavel and Giulia di Rocco and Jorge Lampreia and Juan J Calvete and Alice S. Pereira and Jos{\'e} J. G. Moura and Isabel Moura},
  journal={Journal of inorganic biochemistry},
  year={2004},
  volume={98 5},
  pages={833-40}
}
An orange-coloured protein (ORP) isolated from Desulfovibrio gigas, a sulphate reducer, has been previously shown by extended X-ray absorption fine structure (EXAFS) to contain a novel mixed-metal sulphide cluster of the type [S(2)MoS(2)CuS(2)MoS(2)] [J. Am. Chem. Soc. 122 (2000) 8321]. We report here the purification and the biochemical/spectroscopic characterisation of this novel protein. ORP is a soluble monomeric protein (11.8 kDa). The cluster is non-covalently bound to the polypeptide… CONTINUE READING