Antagonistic property of buprenorphine for putative ϵ-opioid receptor-mediated G-protein activation by β-endorphin in pons/medulla of the μ-opioid receptor knockout mouse

@article{Mizoguchi2002AntagonisticPO,
  title={Antagonistic property of buprenorphine for putative ϵ-opioid receptor-mediated G-protein activation by $\beta$-endorphin in pons/medulla of the $\mu$-opioid receptor knockout mouse},
  author={Hirokazu Mizoguchi and H.-e. Wu and Minoru Narita and F. Scott Hall and Ichiro Sora and George R. Uhl and Hiroshi Nagase and Leon F. Tseng},
  journal={Neuroscience},
  year={2002},
  volume={115},
  pages={715-721}
}
Abstract β-Endorphin is a non-selective opioid peptide which binds μ-, δ- and putative ϵ (β-endorphin-sensitive non-μ-, non-δ- and non-κ 1 -)-opioid receptors. We have previously reported that β-endorphin-produced G-protein activation is mediated by the stimulation of both μ- and putative ϵ-opioid receptors. The present study was designed to further characterize this putative ϵ-opioid receptor-mediated G-protein activation in the pons/medulla membrane obtained from mice lacking μ-opioid… Expand
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Buprenorphine Blocks ϵ- and μ-Opioid Receptor-Mediated Antinociception in the Mouse
TLDR
It is concluded that buprenorphine, at small doses, blocks ϵ-opioid receptor-mediated β-endorphin-induced antinociception and μ-opionceptor-mediated DAMGO-inducedAntagonistic properties, and at high doses produces a μ-OPioid receptors-mediated antinOCiception. Expand
Loss of μ-opioid receptor-mediated G-protein activation in the pons/medulla of mice lacking the exons 2 and 3 of μ-opioid receptor gene
TLDR
The present results suggest that MOR that is created from the sequences encoded with exons 2 and 3 of the MOR gene, as has been previously observed in studies of mice lacking exon 1 of this gene, may be another critical target for the activation of G-protein by MOR agonists in the mouse pons/medulla. Expand
Differential mechanism of G-protein activation induced by endogenous mu-opioid peptides, endomorphin and beta-endorphin.
TLDR
The subject of the present review is to focus on the differential mechanism underlying G-protein activation induced by these mu-opioid peptides using the [35S]GTPgammaS binding assay. Expand
Non-opioid actions of opioid peptides.
TLDR
This work has investigated Met-enkephalin-Arg-Phe (MERF) and its analogues by the means of direct and indirect radioligand binding assays and found that in addition to kappa(2) and delta-opioid receptors, MERF can act also through sigma(2)- or probably via FMRF-NH( 2) receptors in rat cerebellum. Expand
In vivo and in vitro characterization of naltrindole-derived ligands at the κ-opioid receptor
TLDR
Interestingly, while 5’-AMN and 5'-MABN were not κ-selective, both compounds did show significant antidepressant- and anxiolytic-like effects at 7–14 days post-injection in mice, contrary to the hypothesis. Expand
No evidence for G-protein-coupled epsilon receptor in the brain of triple opioid receptor knockout mouse.
TLDR
It is concluded that the brain epsilon site involves mu, delta and/or kappa receptors, possibly coupled to nonclassical G proteins. Expand
Effects of beta-endorphin on endothelial/monocytic endothelin-1 and nitric oxide release mediated by mu1-opioid receptors: a potential link between stress and endothelial dysfunction?
TLDR
A role of beta-Endorphin is hypothesized in the pathogenesis of stress-induced endothelial dysfunction through peripherally circulating beta-endorphin, which may offset the balance of vasoactive mediators, leading to an unopposed vasoconstriction. Expand
Recent Advances in the Search for the -Opioidergic System
The newly discovered endogenous μ-opioid receptor (MOP-R) ligands endomorphin-1 (EM-1) and -2 (EM-2) exhibit the highest specificity and affinity for the MOP-R of any endogenous substance so far… Expand
Differential effects of gestational buprenorphine, naloxone, and methadone on mesolimbic mu opioid and ORL1 receptor G protein coupling.
TLDR
These findings on mu opioid receptor (MOR) GTP-binding regulatory protein (G protein) coupling and its gender dependency are consistent with earlier studies on mu receptor binding adaptation induced by buprenorphine in dams and neonatal rats after in utero treatment regimens, and they extend the gestational effects of this opiate to mu and N/OFQ receptor functionality. Expand
Selective impairment of spinal mu-opioid receptor mechanism by plasticity of serotonergic facilitation mediated by 5-HT2A and 5-HT2B receptors
TLDR
Results indicate that plasticity of spinal serotonergic neurotransmission can selectively reduce spinal MOR mechanisms via 5‐HT2A and 5‐ HT2B receptors, including upregulation of the latter and increased expression in dorsal horn neurons containing MOR. Expand
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