Another potential artifact in the study of nucleosome phasing by chromatin digestion with micrococcal nuclease.

@article{McGhee1983AnotherPA,
  title={Another potential artifact in the study of nucleosome phasing by chromatin digestion with micrococcal nuclease.},
  author={James D McGhee and Gary Felsenfeld},
  journal={Cell},
  year={1983},
  volume={32 4},
  pages={1205-15}
}
We show that, contrary to expectations, restriction enzyme cleavage of chicken erythrocyte nucleosome core particle DNA generates a series of distinct subnucleosome fragments. These fragments do not result from bulk nucleosome phasing in vivo, but arise from micrococcal nuclease cleavages internal to the core particle, at roughly 10-base pair intervals and at AT-rich sequences. Those 145-base pair DNA fragments remaining intact are a biased population in which the guanine content can fluctuate… CONTINUE READING