Anopheles culicifacies sibling species in Odisha, eastern India: First appearance of Anopheles culicifacies E and its vectorial role in malaria transmission

  title={Anopheles culicifacies sibling species in Odisha, eastern India: First appearance of Anopheles culicifacies E and its vectorial role in malaria transmission},
  author={Mumani Das and Biswadeep Das and Aparna Priyadarshini Patra and Hare K Tripathy and Namita Mohapatra and Santanu Kumar Kar and Rupenangshu Kumar Hazra},
  journal={Tropical Medicine \& International Health},
OBJECTIVE To identify the Anopheles culicifacies sibling species complex and study their vectorial role in malaria endemic regions of Odisha. [] Key MethodMETHODS Mosquitoes were collected from 6 malaria endemic districts using standard entomological collection methods. An. culicifacies sibling species were identified by multiplex polymerase chain reaction (PCR) using cytochrome oxidase subunit II (COII) region of mitochondrial DNA.

Distribution of Anopheles culicifacies and Detection of its Sibling Species E from Madhya Pradesh: Central India

This is the first report to detect species E from Madhya Pradesh region which necessitate for reconsideration of species distribution of each An.

Biology & control of Anopheles culicifacies Giles 1901

Current priority areas of research should include in-depth vector biology and control in problem pockets, preparation of malaria-risk maps for focused and selective interventions, monitoring insecticide resistance, cross-border initiative and data sharing, and coordinated control efforts for achieving transmission reduction, and control of drug-resistant malaria.

Population Dynamics and Insecticide Susceptibility of Anopheles culicifacies in Malaria Endemic Districts of Chhattisgarh, India

A study in tribal areas of Chhattisgarh state to investigate the seasonal and ecotype-wise distribution, breeding habits, sibling species composition, insecticide susceptibility, and role in the transmission of the local vector population found An.

Biology & control of Anopheles culicifacies Giles 1901 Biology & control of Anopheles culicifacies Giles 1901

This work states that Anopheles culicifacies is responsible for epidemic malaria in its range of distribution and may cause intense malaria transmission in an estimated 75 million tribal population in India.

Studies on some species of Indian Anopheles based on mitochondrial gene COII (Diptera: Culicidae: Anophelinae)

This study analysed a partial sequence of 183 bp (approx.) of COII gene for four species of subfamily Anophelinae of Anopheles and found that the interspecific distance between these Anophele species was calculated to be 9.88% while intraspecific distance between populations of same species was 0.65%.

Bionomics of Anopheles fluviatilis and Anopheles culicifacies (Diptera: Culicidae) in Relation to Malaria Transmission in East-Central India

Abstract The southern districts of Odisha State in east-central India have been highly endemic for falciparum malaria for many decades. However, there is no adequate information on the abundance of


These entomologicalindicators such as vector density, distribution, biology and bionomics as well as their vectorial attributes are important parameters to measure the pattern and intensity of malariatransmission.

Relative Abundance and Plasmodium Infection Rates of Malaria Vectors in and around Jabalpur, a Malaria Endemic Region in Madhya Pradesh State, Central India

This study provides crucial information on the prevalence of An.



Vector Genetics in Malaria Control

Cytogenetic analysis of polytene chromosomes revealed that A. culicifacies is a complex of five sibling species designated as A, B, C, D, and E, which differ in their larval and adult biology, response to insecticides, and capacity to transmit the disease.

Development and evaluation of a single‐step multiplex PCR to differentiate the aquatic stages of morphologically similar Aedes (subgenus: Stegomyia) species

Objective  To develop a single‐step multiplex PCR to differentiate the aquatic stages of Aedes aegypti, Aedes albopictus and Aedes vittatus collected from different breeding spots in arbovirus

Anopheles culicifacies breeding in brackish waters in Sri Lanka and implications for malaria control

The study demonstrates the ability of An.

Distribution of sibling species of Anopheles culicifacies s.l. and Anopheles fluviatilis s.l. and their vectorial capacity in eight different malaria endemic districts of Orissa, India.

The study observed that the co-existence of potential vector sibling species of An.

Chromosomal evidence for sibling species of the malaria vector Anopheles (Cellia) culicifacies Giles.

The ovarian polytene chromosomes of adult Anopheles culicifacies from natural populations in Pakistan, India and Sri Lanka, and from four laboratory colonies are compared with the published cytological map for this taxon, finding both types of Xs are found in a natural population near New Delhi in the apparent absence of heterozygotes.

Confirmation of Anopheles varuna in vietnam, previously misidentified and mistargeted as the malaria vector Anopheles minimus.

Despite the fact that several potential secondary vectors were found during the study, the primary target for vector control in the region should be An.

Identification of all members of the anopheles culicifacies complex using allele-specific polymerase chain reaction assays.

This assay system is the first, and the best available at present to distinguish all sibling species and especially to discriminate non-vector, species B from all the vector species, A, C, D, and E, of the An.

Evidence for a new malaria vector species, species E, within the Anopheles culicifacies complex (Diptera: Culicidae).

It is proposed that the nonvector population with acrocentric Y-chromosome sons retain the original designation of species B and that the vector population with the submetacentric Y- chromosome sons be designated as species E, a new species.

An allele-specific polymerase chain reaction assay for the differentiation of members of theAnopheles culicifacies complex

An allele-specific polymerase chain reaction (ASPCR) assay targeted to the D3 domain of 28S ribosomal DNA was developed and correctly differentiates An.