Anomeric specificity of hexokinase and glucokinase activities in liver and insulin-producing cells.

@article{Sener1985AnomericSO,
  title={Anomeric specificity of hexokinase and glucokinase activities in liver and insulin-producing cells.},
  author={Abdullah Sener and M. H. Giroix and S. P. Dufrane and Willy Jean Malaisse},
  journal={The Biochemical journal},
  year={1985},
  volume={230 2},
  pages={345-51}
}
Conflicting data have been reported concerning the anomeric specificity of glucokinase. In the present study, liver hexokinase (Km for D-glucose 0.4 mM) displayed a higher affinity for but lower Vmax. with alpha- than with beta-D-glucose. The velocity of the reaction catalysed by liver glucokinase was higher with with beta- than with alpha-D-glucose, whatever the glucose concentration. The apparent Km of glucokinase was somewhat lower, however, with alpha- than with beta-D-glucose. Comparable… CONTINUE READING