Anomalous transport resolved in space and time by fluorescence correlation spectroscopy.
@article{Hofling2011AnomalousTR, title={Anomalous transport resolved in space and time by fluorescence correlation spectroscopy.}, author={Felix Hofling and Karl-Ulrich Bamberg and Thomas Franosch}, journal={Soft Matter}, year={2011}, volume={7}, pages={1358-1363} }
A ubiquitous observation in crowded cell membranes is that molecular transport does not follow Fickian diffusion but exhibits subdiffusion. The microscopic origin of such a behaviour is not understood and highly debated. Here we discuss the spatio-temporal dynamics for two models of subdiffusion: fractional Brownian motion and hindered motion due to immobile obstacles. We show that the different microscopic mechanisms can be distinguished using fluorescence correlation spectroscopy (FCS) by…
33 Citations
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References
SHOWING 1-10 OF 59 REFERENCES
Fluorescence correlation spectroscopy diffusion laws to probe the submicron cell membrane organization.
- PhysicsBiophysical journal
- 2005
It is shown that fluorescence correlation spectroscopy (FCS) measurements at different spatial scales enable distinguishing between different submicron confinement models, and the so-called FCS diffusion law is introduced.
Anomalous protein diffusion in living cells as seen by fluorescence correlation spectroscopy.
- PhysicsBiophysical journal
- 2003
Development of anomalous diffusion among crowding proteins
- Biology
- 2010
A systematic study where proteins bound to supported lipid membranes diffuse freely in two dimensions, but are increasingly hindered by the presence of other like proteins, to investigate the onset of anomalous diffusion.
New fluorescence correlation spectroscopy enabling direct observation of spatiotemporal dependence of diffusion constants as an evidence of anomalous transport in extracellular matrices.
- PhysicsBiophysical journal
- 2005
This new method, namely, sampling-volume-controlled (SVC)-FCS, was applied to the analysis of hyaluronan aqueous solutions and indicates that moderate ASD may occur even in the presence of a small amount of HA in ECM.
Fluorescence correlation spectroscopy and its potential for intracellular applications
- ChemistryCell Biochemistry and Biophysics
- 2007
The scope of this review is to introduce the FCS technique in particular to the reader with biological background, searching for new methods for a precise quantification of physical parameters governing cellular mechanisms and dynamics, especially if high sensitivity and fast dynamic resolution are required.
Anomalous diffusion of proteins due to molecular crowding.
- BiologyBiophysical journal
- 2005
Observations indicate that protein diffusion in the cell cytoplasm and nucleus should be anomalous as well, with consequences for measurements of solute diffusion coefficients in cells and for the modeling of cellular processes relying on diffusion.
Diffusion analysis within single nanometric apertures reveals the ultrafine cell membrane organization.
- ChemistryBiophysical journal
- 2007
A new methodology to probe the plasma membrane organization of living cells at the nanometric scale by performing fluorescence correlation spectroscopy with increasing aperture sizes and extracting information on the diffusion process from the whole set of data is described.
Elucidating the origin of anomalous diffusion in crowded fluids.
- PhysicsPhysical review letters
- 2009
It is shown by means of fluorescence correlation spectroscopy and simulations that the properties of crowding-induced subdiffusion are consistent with the predictions for fractional Brownian motion or obstructed (percolationlike) diffusion, both of which have stationary increments.
Critical dynamics of ballistic and Brownian particles in a heterogeneous environment.
- PhysicsThe Journal of chemical physics
- 2008
The dynamic properties of a classical tracer particle in a random, disordered medium are investigated close to the localization transition and suggest that apparent power laws with varying exponents generically occur and dominate experimentally accessible time windows as soon as the heterogeneities cover a decade in length scale.
Biological and chemical applications of fluorescence correlation spectroscopy: a review.
- ChemistryBiochemistry
- 2002
Technological advances in detectors, autocorrelation electronics, and confocal microscopy were incorporated into FCS, and the ability of FCS to resolve multiple species with equivalent diffusion properties was extended by probability analysis of fluorescence intensity distributions.