Annexin V binding assay as a tool to measure apoptosis in differentiated neuronal cells

@article{Schutte1998AnnexinVB,
  title={Annexin V binding assay as a tool to measure apoptosis in differentiated neuronal cells},
  author={Bert Schutte and Rony Nuydens and Hugo Geerts and F. Ramaekers},
  journal={Journal of Neuroscience Methods},
  year={1998},
  volume={86},
  pages={63-69}
}
  • Bert Schutte, Rony Nuydens, +1 author F. Ramaekers
  • Published in
    Journal of Neuroscience…
    1998
  • Medicine, Biology
  • We describe a rapid and reliable method to quantitate the extent of apoptosis in neuronal cell cultures. Based on their annexin V-affinity, resulting from phosphatidylserine (PS) exposure at the outer leaflet of the plasma membrane, apoptotic cells can be distinguished from annexin V-negative living cells, by using microscopic and flow cytometric procedures. When combined with propidium iodide (PI) the double labeling procedure allows a further distinction of necrotic (annexin V+/PI… CONTINUE READING

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