Annexin 1 and its bioactive peptide inhibit neutrophil-endothelium interactions under flow: indication of distinct receptor involvement.

@article{Hayhoe2006Annexin1A,
  title={Annexin 1 and its bioactive peptide inhibit neutrophil-endothelium interactions under flow: indication of distinct receptor involvement.},
  author={Richard P. G. Hayhoe and Ahmad M. Kamal and Egle Solito and Roderick John Flower and Dianne Cooper and Mauro Perretti},
  journal={Blood},
  year={2006},
  volume={107 5},
  pages={
          2123-30
        }
}
We have tested the effects of annexin 1 (ANXA1) and its N-terminal peptide Ac2-26 on polymorphonuclear leukocyte (PMN) recruitment under flow. Differential effects of the full-length protein and its peptide were observed; ANXA1 inhibited firm adhesion of human PMNs, while Ac2-26 significantly attenuated capture and rolling without effect on firm adhesion. Analysis of the effects of ANXA1 and Ac2-26 on PMN adhesion molecule expression supported the flow chamber results, with Ac2-26 but not ANXA1… 
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neutrophil-derived microparticles Annexin 1 mediates the rapid anti-inflammatory effects of
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In vivo support is provided to the hypothesis that endogenous AnxA1 is an essential effector of endogenous anti-inflammation and an ultrastructural indication that this mediator interacts with Fpr2 in murine neutrophils is provided.
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A novel AnxA1-dependent mechanism behind the inhibitory properties of estrogen on PMN activation is unveiled, describing a novel phenotype with a conceivable impact on the vasculoprotective effects of this hormone.
Annexin I Regulates SKCO-15 Cell Invasion by Signaling through Formyl Peptide Receptors*
TLDR
The findings in this study suggest that activation of nFPRs stimulates epithelial cell motility important in the development of metastasis as well as wound healing and support an autocrine/paracrine role for membrane AnxA1 in stimulating SKCO-15 cell migration through nF PR activation.
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TLDR
These findings identify CR-AnxA12-48 as a new ALX agonist that regulates phagocyte responses and displays tissue-protective actions.
Anti-Inflammatory Role of the Murine Formyl-Peptide Receptor 2: Ligand-Specific Effects on Leukocyte Responses and Experimental Inflammation
TLDR
The creation of a novel mouse colony in which the murine FprL1 FPR2 homologue, Fpr2, has been deleted is reported and its use to explore the biology of this receptor is described, concluding that Fpr 2 is an anti-inflammatory receptor that serves varied regulatory functions during the host defense response.
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References

SHOWING 1-10 OF 65 REFERENCES
Leukocyte antiadhesive actions of annexin 1: ALXR- and FPR-related anti-inflammatory mechanisms.
TLDR
The present findings demonstrate that endogenous regulatory autocoids such as lipoxin A(4) and annexin 1-derived peptides function to disengage adherent cells during cell-cell interactions.
A novel calcium‐dependent proapoptotic effect of annexin 1 on human neutrophils
TLDR
It is proposed that the new proapoptotic effect reported here may be part of the spectrum of ANXA1‐mediated events involved in the resolution of acute inflammation.
An Annexin 1 N-Terminal Peptide Activates Leukocytes by Triggering Different Members of the Formyl Peptide Receptor Family1
TLDR
It is shown that a peptide derived from the N-terminal domain of the anti-inflammatory protein annexin 1 (lipocortin 1) can activate all three FPR family members at similar concentrations and indicate that annexin1 participates in regulating leukocyte emigration into inflamed tissue by activating and desensitizing different receptors of the F PR family.
Selective inhibition of neutrophil function by a peptide derived from lipocortin 1 N-terminus.
Annexin 1 Binds to U937 Monocytic Cells and Inhibits Their Adhesion to Microvascular Endothelium: Involvement of the α4β1 Integrin1
TLDR
The results indicate that ANX1 plays an important physiological role in modulating monocyte firm adhesion to the endothelium.
Neutrophil NADPH‐oxidase activation by an annexin AI peptide is transduced by the formyl peptide receptor (FPR), whereas an inhibitory signal is generated independently of the FPR family receptors
TLDR
It is reported that an annexin AI peptide (Ac9–25) activates, as well as inhibits, the neutrophil release of superoxide anions and the inhibitory signal being transduced by a not‐yet identified receptor distinct from FPR and FPRL1.
A Potential Role for Annexin 1 as a Physiologic Mediator of Glucocorticoid-Induced L-Selectin Shedding from Myeloid Cells1
TLDR
It is shown that ANX1 induces a dose- and time-dependent decrease in L-selectin expression on both peripheral blood neutrophils and monocytes but has no effect on lymphocytes, which may provide the basis for further understanding of mechanisms involved in the down-regulation of inflammatory responses.
A novel ligand of the formyl peptide receptor: annexin I regulates neutrophil extravasation by interacting with the FPR.
Anti-inflammatory actions of lipoxin A(4) stable analogs are demonstrable in human whole blood: modulation of leukocyte adhesion molecules and inhibition of neutrophil-endothelial interactions.
TLDR
Results indicate that LXA(4) stable analogs modulate expression of both L-selectin and CD11/CD18 on resting and immunostimulated leukocytes and inhibit neutrophil adhesion to HCAEC by attenuating CD11-CD18 expression.
Identification and characterization of an endogenous chemotactic ligand specific for FPRL2
TLDR
F2L appears as a new natural chemoattractant peptide for DCs and monocytes, and the first potent and specific agonist of FPRL2, the third member of this structural family of chemoATTractant receptors.
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