Anion exchange SPE and liquid chromatography-tandem mass spectrometry in GHB analysis.

@article{Elian2011AnionES,
  title={Anion exchange SPE and liquid chromatography-tandem mass spectrometry in GHB analysis.},
  author={Albert A. Elian and Jeffery Hackett},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2011},
  volume={879 31},
  pages={3752-8}
}
In this study, the extraction of γ-hydroxybutyrate (GHB) from urine using solid-phase extraction (SPE) is described. SPE was performed on anion exchange columns after samples of urine had been diluted with de-ionized water. After application of the diluted samples containing GHB-d(6) as an internal standard, the sorbent was washed with deionized water and methanol and dried. The GHB was eluted from the SPE column with a solvent consisting of methanol containing 6% glacial acetic acid. The… CONTINUE READING