Anion currents in yeast K+ transporters (TRK) characterize a structural homologue of ligand-gated ion channels

@article{Rivetta2011AnionCI,
  title={Anion currents in yeast K+ transporters (TRK) characterize a structural homologue of ligand-gated ion channels},
  author={Alberto Rivetta and Teruo Kuroda and Clifford L Slayman},
  journal={Pfl{\"u}gers Archiv - European Journal of Physiology},
  year={2011},
  volume={462},
  pages={315-330}
}
Patch clamp studies of the potassium-transport proteins TRK1,2 in Saccharomyces cerevisiae have revealed large chloride efflux currents: at clamp voltages negative to −100 mV, and intracellular chloride concentrations >10 mM (J. Membr. Biol. 198:177, 2004). Stationary-state current-voltage analysis led to an in-series two-barrier model for chloride activation: the lower barrier (α) being 10–13 kcal/mol located ∼30% into the membrane from the cytoplasmic surface; and the higher one (β) being 12… CONTINUE READING