Animal transgenesis: state of the art and applications

  title={Animal transgenesis: state of the art and applications},
  author={Eduardo de Oliveira Melo and Aurea Maria Oliveira Canavessi and Maur{\'i}cio Machaim Franco and Rodolfo Rumpf},
  journal={Journal of Applied Genetics},
There is a constant expectation for fast improvement of livestock production and human health care products. The advent of DNA recombinant technology and the possibility of gene transfer between organisms of distinct species, or even distinct phylogenic kingdoms, has opened a wide range of possibilities. Nowadays we can produce human insulin in bacteria or human coagulation factors in cattle milk. The recent advances in gene transfer, animal cloning, and assisted reproductive techniques have… 

Transgenesis techniques and its application in poultry production

Transgenic technologies may revolutionalize the production of poultry birds with improved meat quality, low fats, low cholesterol as well as disease resistance in addition to production of biopharmaceutical products with efficiencies far greater than any conventional breeding methods.

Transgenic mammalian species, generated by somatic cell cloning, in biomedicine, biopharmaceutical industry and human nutrition/dietetics--recent achievements.

An overall survey of the state of the art in investigations which provide the current knowledge for deciphering the possibilities of practical application of the transgenic mammalian species generated by somatic cell cloning in biomedicine, the biopharmaceutical industry, human nutrition/dietetics and agriculture is presented.

Genetic Modification of Domestic Animals for Agriculture and Biomedical Applications

The recent progress and technical route of the cell-based method of transgenesis followed by somatic cell nuclear transfer by SCNT is reviewed and the newly emerging methods to enrich the gene targeting frequency of somatic cells are discussed.

Review on Applications of Genetic Engineering And Cloning in Farm animals

Through the biotechnology of gene therapy, scientists are making efforts at curing genetic diseases by attempting to replace defective genes with the correct version and also used to produce more effective and efficient vaccines, therapeutic antibodies, antibiotics and other pharmaceuticals.

Transgenic Animal Production and Applications

Use of transgenic animals will provide solutions for disease model, drug research, food production and improved disease resistance, and their potential applications are summarized.

The possibilities of practical application of transgenic mammalian species generated by somatic cell cloning in pharmacology, veterinary medicine and xenotransplantology.

Current knowledge deciphering the possibilities of using transgenic specimens created by somatic cell nuclear transfer in medical pharmacology, veterinary medicine, agriculture, transplantational medicine and immunology are overviewed.

Transgenic farm animals: the status of research and prospects

Methods for the production of transgenic farm animals and their advantages and disadvantages are reviewed, including the creation of animals with altered metabolism for higher quality and productivity, animals genetically resistant to infectious diseases, and producers of biologically active recombinant proteins.

The Role of Recombinant Sperm Technology in Enhancing Mammalian Transgenesis

This review aims to pave the way for every effort to enable the researchers to undergo the transgenesis experiments in the routine laboratories by testing the validity of the most modern enhancement approaches suggested on the original SMGT.

Genetic Engineering and Its Application in Animal Breeding: Review

  • T. Alemneh
  • Biology
    Archives in Biomedical Engineering & Biotechnology
  • 2020
The objectives of this review were to overview on the genetic engineering and its application in animal breeding, and to highlight the general concepts, techniques, types, importance and limitations of genetic engineering inAnimal breeding.



The contribution of farm animals to human health.

Production of bioproducts through the use of transgenic animal models.

  • C. Keefer
  • Biology
    Animal reproduction science
  • 2004

Transgenic dairy cattle: genetic engineering on a large scale.

The ability to modify mammary-specific genes provides an opportunity to pursue several distinctly different avenues of research, one of which is genetic engineering of the bovine mammary gland to alter the composition of milk destined for human consumption.

Genetic engineering of livestock.

The ability to produce pigs exhibiting only the beneficial, growth-promoting effects of growth hormone by a transgenic approach may require better control of transgene expression, a different genetic background, or a modified husbandry regimen.

Production of gene-targeted sheep by nuclear transfer from cultured somatic cells

Efficient and reproducible gene targeting in fetal fibroblasts to place a therapeutic transgene at the ovine α1(I) procollagen (COL1A1) locus is described and the production of live sheep by nuclear transfer is described.

Isolation of transfected fibroblast clones for use in nuclear transfer and transgene detection in cattle embryos.

This approach provided a reliable method for isolating transfected mammalian cells and for diagnosing the incorporation of desirable vectors in NT embryos and can reduce the time and cost of transgenic livestock production.

Toward altering milk composition by genetic manipulation: current status and challenges.

The utility of genetically engineered cattle as a means of altering milk composition to improve the functional properties of milk, increasing marketability and impact on milk quality, attributes of novel dairy products, and human health is explored.

Mammary expression of new genes to combat mastitis

The goal is to enhance mastitis resistance of dairy cows by enabling the cells of the mammary gland to secrete additional antibacterial proteins, including lysostaphin, which has potent anti-staphylococcal activity.

Large scale production of recombinant human lactoferrin in the milk of transgenic cows

Although natural hLF from human milk and rhLF underwent differential N-linked glycosylation, they were equally effective in three different in vivo infection models employing immunocompetent and leukocytopenic mice, and showed similar localization at sites of infection.

Resource requirements for transgenic livestock research.

  • G. Seidel
  • Biology
    Journal of animal science
  • 1993
Three broad areas of expertise are required for successful transgenic projects: molecular biology, embryo micromanipulation/reproductive physiology, and knowledge of the biology of the tissues/systems affected by the transgene.