Animal Models of Entamoeba histolytica Infection

  title={Animal Models of Entamoeba histolytica Infection},
  author={S. Stanley and Tonghai Zhang and K. Seydel},
Publisher Summary This chapter presents animal models of Entamoeba histolytica infection. Male or female CB-17 SCID mice, aged 6–10 weeks old, are used as xenograft recipients. Since maturation of the intestinal xenograft requires at least 10 weeks post-surgery, use of younger mice are recommended. All SCID mice are screened for the "leaky" phenotype at the age of five weeks by measuring serum antibodies by using an enzyme-linked immunosorbent (ELISA) assay. SCID mice are housed in a sterile… Expand
2 Citations
Expression of Amoebapores Is Required for Full Expression of Entamoeba histolytica Virulence in Amebic Liver Abscess but Is Not Necessary for the Induction of Inflammation or Tissue Damage in Amebic Colitis
It is demonstrated that amoebapore expression is required for full virulence in the SCID mouse model of amebic liver abscess, but E. histolytica trophozoites that do not express amoEBapore-A can still cause inflammation and tissue damage in infected human colonic xenografts. Expand
Water-Soluble Ruthenium (II) Chiral Heteroleptic Complexes with Amoebicidal in Vitro and in Vivo Activity.
Three water-soluble Ru(II) chiral heteroleptic coordination compounds inhibit the trophozoite proliferation in amoebic liver abscess induced in hamster, and are proposed as promising candidates as antiparasitic agents. Expand


Intestinal invasive amebiasis: an experimental model in rodents using axenic or monoxenic strains of Entamoeba histolytica.
A new experimental model for the production of early stages of invasive intestinal amebiasis in hamsters or guinea pigs with axenic or monoxenic cultures of Entamoeba histolytica of the HM1:IMSSExpand
Intestinal amebiasis: histopathologic features in experimentally infected mice.
The view that tissue invasion by trophozoites is not necessarily required for ulcerative disease to occur is supported, suggesting a role for toxic factors released by amebae. Expand
A severe combined immunodeficient (SCID) mouse model for infection with Entamoeba histolytica
It is demonstrated that lymphocyte-based immunity is important in protection against amebic liver abscess, and that anti-E. Expand
The experimental infection of Macaca mulatta with human strains of Entamoeba histolytica.
  • M. Miller
  • Biology, Medicine
  • The American journal of tropical medicine and hygiene
  • 1952
While it has been shown by many investigators that this parasite can be transmitted to a wide variety of hosts, in none of these can the infection be considered a normal one, and a proper understanding of the host-parasite relationships of E. histolytica the parasite must be studied in its normal host. Expand
Protection against amebic liver abscess formation in the severe combined immunodeficient mouse by human anti-amebic antibodies.
It is demonstrated that human anti-amebic antibodies are capable of exerting a protective effect in an animal model of amebic liver abscess formation and are able to reduce the mean abscess size in animals. Expand
Role of the Entamoeba histolytica cysteine proteinase in amebic liver abscess formation in severe combined immunodeficient mice
An important role for a functional cysteine proteinase in amebic liver abscess formation is suggested in mice with severe combined immunodeficiency (SCID mice). Expand
Entamoeba histolytica and Entamoeba dispar are distinct species; clinical, epidemiological and serological evidence.
  • T. Jackson
  • Biology, Medicine
  • International journal for parasitology
  • 1998
The name of the causative organism of invasive amoebiasis, Entamoeba histolytica, was first introduced in 1903, but the concept of two morphologically identical species gained increasing acceptance 50-60 years later when technology became available to investigate this anomaly. Expand
Antibodies to the serine rich Entamoeba histolytica protein (SREHP) prevent amoebic liver abscess in severe combined immunodeficient (SCID) mice
It is demonstrated that antibodies to a recombinant version of the amoebic SREHP molecule can protect against amoeebic liver abscess, and suggested the recombinant S REHP molecule should be considered as a possible vaccine candidate to prevent amoEBic Liver abscess. Expand
Effect of corticosteroid and irradiation on caecal amoebic infection in rats.
Albino rats (Wistar strain) were pretreated with corticosteroid, irradiation or both to study the effect on the outcome of amoebic infection given intracaecally and it is thought that once the amoEBic infection is established, isoebae become better established for penetration in the immuno-depressed host. Expand
Identification of an Epitope on the Entamoeba histolytica 170-kD Lectin Conferring Antibody-mediated Protection against Invasive Amebiasis
Rec recombinant proteins corresponding to four distinct domains of the molecule, and synthetic peptides are used to localize both protective and exacerbative epitopes of the heavy chain subunit of the lectin, showing that protective immunity after vaccination can be correlated with the development of an antibody response to a region of 25 amino acid residues of the Lectin. Expand