Animal Experimentation: Vero Cells, But Not Oviductal Cells, Increase the Hatching Frequency and Total Cell Count of Mouse Blastocysts Partly by Changing Energy Substrate Concentrations in Culture Medium

Abstract

Purpose: To investigate the embryotrophic mechanisms of Vero and oviductal cells coculture. Methods: Mouse embryos were cultured in Chatot, Ziomek, and Bavister medium (CZB), in modified CZB media (MM) with nutrient concentrations adjusted to that found in conditioned media after different periods of Vero cells or oviductal cells culture, in reconstituted… (More)
DOI: 10.1023/A:1011910125079

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