Angiotensin-converting enzyme from rabbit pulmonary particles.

  title={Angiotensin-converting enzyme from rabbit pulmonary particles.},
  author={Richard L. Soffer and Rajabnejad Mohammad Reza and Penny R. Caldwell},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={71 5},
Angiotensin-converting enzyme has been solubilized from rabbit pulmonary particles and purified to homogeneity. The molecular weight of the native enzyme was estimated to be about 136,000 by glycerol gradient centrifugation, and a value of 140,000 was obtained for the reduced denatured protein by disc-gel electrophoresis in the presence of sodium dodecyl sulfate and 2-mercaptoethanol. The enzyme was found to be a glycoprotein with carbohydrate accounting for approximately 16% of its dry weight… CONTINUE READING