Angiotensin converting enzyme: method of assay and partial purification.

@article{Dorer1970AngiotensinCE,
  title={Angiotensin converting enzyme: method of assay and partial purification.},
  author={Frederic E. Dorer and Leonard T. Skeggs and Joseph R. Kahn and Kenneth E. Lentz and Melvin Levine},
  journal={Analytical biochemistry},
  year={1970},
  volume={33 1},
  pages={
          102-13
        }
}

Partial purification and properties of angiotensin I converting enzyme from rabbit plasma.

Angiotensin I converting enzyme was purified from rabbit plasma by means of ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography and SephadeX G-200 gel filtration and the specific activity of the enzyme was elevated 63 fold by the purification method, suggesting that the enzyme hydrolyses the Phe-His bond of angiotens in I.

Human plasma converting enzyme.

Angiotensin-converting enzyme from porcine plasma.

Purification and Properties of Angiotensin‐Converting Enzyme from Hog Lung

It would, therefore, appear that a single enzyme, purified in the described manner, hydrolyzes both angiotensin I and hippurylglycylglycine.

Purification and characterization of angiotensin converting enzyme‐II from bovine seminal plasma

Angiotensin converting enzyme from bovine seminal plasma was shown to exist in two distinct forms and was inhibited by some heavy metal ions and required disulfide linkages at its active site.

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The assay is sufficiently sensitive to measure renin in the plasma of all normal rabbits and can be extended to measure much lower activities, such as the percentage release of the angiotensin content/hr.

THE PREPARATION AND FUNCTION OF THE HYPERTENSIN-CONVERTING ENZYME

It has been shown by use of isolated, perfused rat kidneys that hypertensin II is a potent vasoconstrictor substance while hypertensin I is not. Hence it would appear that in intact animals the

Conversion of Angiotensin I to Angiotensin II by Cell-free Extracts of Dog Lung

This communication describes some of the enzymes in extracts of dog lung that metabolize angiotensins I and II and particularly those catalysing the conversion ofAngiotensin I to angiotENSin II (“converting enzyme”) and the enzyme(s) responsible for the inactivation of ang Elliotensin II and angiotsin I are referred to as “destroying enzyme’.

THE AMINO ACID COMPOSITION OF HYPERTENSIN II AND ITS BIOCHEMICAL RELATIONSHIP TO HYPERTENSIN I

It was concluded that the conversion of hypertensin I by the plasma hypertensIn converting enzyme involved hydrolysis of the phenylalanyl-histidine bond to form hypertens in II and histidylleucine.

THE EXISTENCE OF TWO FORMS OF HYPERTENSIN

Two types of hypertensin have been demonstrated by means of counter-current distribution and a highly purified preparation containing horse hypertensins I and II caused an elevation of blood pressure when injected into human beings.