Angiotensin I-generating acid endopeptidase activity in neurosecretory vesicles isolated from bovine pituitary

@article{Gainer1984AngiotensinIA,
  title={Angiotensin I-generating acid endopeptidase activity in neurosecretory vesicles isolated from bovine pituitary},
  author={Harold Gainer and James T. Russell and Yoke Peng Loh},
  journal={Neuropeptides},
  year={1984},
  volume={4},
  pages={447-455}
}
Secretory vesicles purified from the neural and intermediate lobes of the bovine pituitary contain acidic endopeptidases which are capable of converting renin tetradecapeptide (RTD) substrate to Angiotensin I (AI). Preliminary characterization of the neurosecretory vesicle (NSV) endopeptidase showed that it had a pH optimum of 4.0, and unlike renin was inactive at pHs greater than 6.0. It is inhibited by 10(-6) M pepstatin A, but not by PMSF, leupeptin, PMBS, or the specific renin inhibitor H… CONTINUE READING